Recombinant protein corresponding to bovine Tumor Necrosis Factor alpha, expressed in Pichia pastoris Description: Bovine Tumor Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response. Biological Activity: ED50 in the range of 10-25ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D. Applications: Suitable for use in In ELISA and Functional assays. Other applications not tested. Storage and Stability: Lyophilized and reconstituted products may be stored at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
