Tumor Necrosis Factor-alpha (TNF-alpha) is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF superfamily of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation and diabetes. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Neutralization studies. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:10 Western Blot: 1:10 Neutralization: biological activity dilutions have to be made according to the amounts TNF-alpha to be inactivated. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, 0.1% BSA.
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