Tumor necrosis factor alpha (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Recombinant mouse TNF-alpha is a soluble 156 amino acid protein (17.3kD) which corresponds to C-terminal extracellular domain of the full length transmembrane protein, expressed in E. coli. Molecular Weight: ~17.3kD (156aa sequence) Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is 0.1ng/ml. Specific Activity: ~1x107units/mg Amino Acid Sequence: MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
