Recombinant protein corresponding to mouse TNF-alpha expressed in E. coli
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Western Blot: 1-5ug/ml (in conjunction with AP-labeled goat Anti-rat) has been found to enable detection of 100ng/lane of recombinant mouse TNFa under reducing conditions. Neutralization: I1904-76UX should be used as an isotype control. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[MP6-XT3]
Purity:
Purified
Form:
Supplied as a liquid in PBS. No preservative added.
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