Recombinant protein corresponding to human TNF-alpha.
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Removal of sodium azide is recommended prior to functional assays. Optimal dilutions to be determined by the researcher. Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63.Ag.8653 myeloma cell line. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[B-C7 3H3096]
Isotype:
IgG1
Purity:
Purified by Ion Exchange chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
* VAT and and shipping costs not included. Errors and price changes excepted
