Tumor Necrosis Factor Receptor 1 (TNFR1, Tumor Necrosis Factor Receptor Superfamily Member 1A, TNF-R1, Tumor Necrosis Factor Receptor Type I, TNF-RI, TNFR-I, p55, p60, CD120a, TNFRSF1A, TNFAR), Rabbit
Biozol Catalog Number:
USB-T9161-07K
Supplier Catalog Number:
T9161-07K
Alternative Catalog Number:
USB-T9161-07K-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
IHC, WB
Immunogen:
Synthetic peptide corresponding to aa195-211, CLPQIENVKGTEDSGTT, in the middle region of human TNFR1.
Tumor necrosis factor receptor 1 (TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Its gene is located on 12p13. The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction. MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein (MAP) kinase (e.g., ERK2) activation and arachidonic acid release. TNFR1-induced apoptosis involves 2 sequential signaling complexes. Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B. In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml Immunohistochemistry (Paraffin): 0.5-1ug/ml. Antigen retrieval by heat is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
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