Recombinant protein corresponding to aa23-257 of human Tumor Necrosis Factor Receptor 2, expressed in E. coli.
Tumor Necrosis Factor Receptor 2, p75/p80 (TNFR2) is present on most cell types and is considered to be an important co-stimulator together with TNF-RI in cellular responses to TNF-alpha, in particular the transmembrane form of TNF-alpha. A dominant role of TNFR2 has been shown in thymocyte activation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNFR1 and TNFR2, which have both distinct and overlapping downstream signalling cascades. Induction of cytotoxicity and other functions are mediated largely via TNFR1. TNFR1 is equally well activated by both the 17kD soluble and 26kD membrane-bound form, whereas TNFR2 is activated only by the membrane bound form of TNF-alpha. Applications: Suitable for use in ELISA, Immunohistochemistry, Functional Assays and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml. The detection limit is 5ng/well (Reducing conditions) Immunohistochemistry (Frozen): 15-25ug/ml Western Blot: 1-2ug/ml. The detection limit is 10ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-83P1: IgG2a Murine Negative Isotype Control Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
