Tumor Necrosis Factor Receptor Associated Factor 3, NT (TRAF-3), Rabbit
Biozol Catalog Number:
USB-T9161-56N
Supplier Catalog Number:
T9161-56N
Alternative Catalog Number:
USB-T9161-56N-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
WB
Immunogen:
Synthetic peptide TRAF3 15aa near the amino terminus of human TRAF3 (Genbank accession No. NP_663777).
Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll- like receptor (IL-1/TLR) superfamily (1). TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1 (2,3). Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-kappaB (4). Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTbetaR (5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ugml Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
