Purified recombinant human soluble TNF Receptor 2.
TNF-RII is expressed on a variety of human tissues including lymphocytes, monocytes, granulocytes, fibroblasts, and endothelial cells. In addition, TNF-RII is released from neutrophils and monocytes after activation. TNF-RII, a member of TNF receptor family, binds to TNF-a and TNF-b. The cytoplasmic domain of TNF-RII interacts with TRAF1 and TRAF2. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: ELISA (Capture): Dilute in an appropriate coating buffer to a concentration of 1-5ug/ml and use 100ul to coat each well. Further dilutions may be made in medium or buffered solution with carrier protein, such as PBS with 0.1-1.0% BSA. Optimal dilutions to be determined by the researcher. Hybridoma: BALB/c splenocytes were fused with NSO mouse myeloma cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[2B2.106]
Isotype:
IgG1
Purity:
Purified by Protein A affinity chromatography
Form:
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
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