N-linked glycosylation is a highly regulated post-translational modification that is involved in protein folding and conformation, oligomerization, sorting, cell-cell interactions, and targeting of proteins to sub- or extra-cellular locations. It is initiated in the endoplastic reticulum with the transfer of a carbohydrate moiety to an asparagine residue within a specific amino acid consensus sequence and then further processed in the Golgi whereupon a mature glycoprotein is exported through the secretory machinery to the plasma membrane. Tunicamycin, a mixture of nucleoside antibiotics, is a specific inhibitor of N-linked glycoslylation that blocks the first step of glycoprotein synthesis thereby inducing protein unfolding. At 500 nM, it can impair the function of several receptor tyrosine kinases, including EGFR, ErbB2, ErbB3, and IGF-IR.1 Also at 500 nM, it radiosensitizes U251 glioma and BXPC3 pancreatic adenocarcinoma cells to chemotherapy.1 Tunicamycin impairs ALK phosphorylation and disrupts pro-survival signaling and cell viability in various neuroblastoma cell lines (IC50s range from 20-500nM).2 Tunicamycin can also inhibit protein palmitoylation.3 Tunicamycin is a mixture of homologuous nucleoside antibiotics. It is useful in glycoprotein research. It is an inhibitor of N-linked glycosylation and the formation of N-glycosidic protein-carbohydrate linkages. It causes the arrest cell cycle in late G1. Tunicamycin has been used to study the effect of N-linked glycosylation of human proton-coupled folate transporter (HsPCFT) in HeLa cells. Tunicamycin has also been used to study the functional effects of coxsackievirus and adenovirus receptor (CAR) glycosylation in COS-7 cells. CAS No: 11089-65-9 Molecular Formula: C39H64N4O16 Molecular Weight: 844.4 Appearance: White to off-white powder Purity: 95% (mixture of congeners) Solubility: DMSO: 50mg/ml Methanol: 5mg/ml Ethanol (95%): 1mg/ml THF: <1mg/ml Dioxane: <1mg/ml DMF: >10mg/ml Pyridine: >10mg/ml Storage and Stability: Powder may be stored at -20C. Stable for 6 months after receipt at -20C For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Warning! Toxic. May be Carcinogenic. Wear gloves/mask when handling product. Avoid contact by all modes of exposure.
Molecular Weight:
844.4
Purity:
95% (mixture of congeners)
Form:
White to off-white powder
CAS Number:
[11089-65-9]
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