TWEAK, Recombinant, Human (TNF-related Weak Inducer of Apoptosis, APO3 Ligand, APO3/DR3 Ligand, APO3L, CD255, DR3LG, FN14, Tumor Necrosis Factor Ligand Superfamily Member 12, TNFSF12, UNQ181/PRO207)
Biozol Catalog Number:
USB-T9185
Supplier Catalog Number:
T9185
Alternative Catalog Number:
USB-T9185-5,USB-T9185-25
Manufacturer:
US Biological
Category:
Molekularbiologie
Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many members of the TNF family, was able to induce interleukin-8 synthesis in a number of cell lines. Recombinant Human TWEAK is a 17.0kD protein, comprising the TNF-like extracellular domain of TWEAK. Biological Activity: The ED50 as determined by the dose-dependent stimulation of IL-8 production by Human PBMC (peripheral blood mononuclear cells) is less than 10ng/ml. TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-y. The ED50 for this effect if between 30-45ng/ml. AA Sequence: MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 6 months after receipt at -20C. Reconstitute with sterile buffer Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA
Purity:
98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
Form:
Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5, 0.1M L-Arg. Reconstitute in 10mM sodium phosphate pH 7.5 to a concentration of 1mg/ml. DO NOT VORTEX
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