Since the original observations that protein tyrosine kinases (PTKs) play critical roles in oncogenic transformation (1,2), it has become clear that both protein tyrosine kinases and phospho-tyrosine phosphatases control a wide range of critical biological processes (3,4). Antibodies specific for phospho-tyrosine (5,6) have been invaluable reagents in these studies. Exceptionally sensitive new tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1:20, 20ul of 50% bead slurry to 200ul of cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[3A45P-Tyr-100]
Isotype:
IgG1
Purity:
Purified.
Form:
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Labeled with Agarose.
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