Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate roliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phosphotyrosine monoclonal antibodies provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:1000 Western Blot: 1:2000, Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clonality:
Monoclonal
Clone Designation:
[11C1089P-Tyr-100]
Isotype:
IgG1
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in 136mM sodium chloride, 2.6mM potassium chloride, 12mM sodium phosphate dibasic, pH 7.4, 2mg/ml BSA 50% glycerol. Labeled with horseradish peroxidase (HRP).
* VAT and and shipping costs not included. Errors and price changes excepted
