Tyrosinase is the principal enzyme required for the synthesis of melanin pigment by melanocytes in mammals. It is also a marker of melanocyte differentiation, and is commonly expressed by malignant melanoma. Tyrosinase immunoreactivity studies in formalin-fixed, paraffin-embedded tissues have demonstrated that the T311 antibody is a specific, sensitive marker for the detection of melanocytic lesions. Immunoreactivity has also been shown to correlate inversely with clinical stage of melanoma progression. Because tyrosinase is recognized by cytotoxic T cells from melanoma patients, it has been proposed as an immunotherapeutic target for melanoma vaccines. The molecular mass of tyrosinase is 60-75kD. However, the processed, glycosylated forms of the protein found in melanosomes have been reported at 70-75kD. The T311 antibody identifies a cluster of proteins at 70-80kD, consistent with previous reports. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: 1ug/ml Western Blot: 1-5ug/ml Note: Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, prior to staining, is recommended for optimal immunohistochemical results with paraffin-embedded tissues. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[T311]
Isotype:
IgG2a
Purity:
Purified by Protein A chromatography
Form:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
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