Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
Tyrosine hydroxylase (TH) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing. Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 on 10ug of PC12 lysates. Immunohistochemistry: 1:100-1:1000 at 4C, 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Reported not suitable for use on paraffin embedded, human tissue. Immunofluorescence: 1:100-1:1000, Fixed with 4% PFA overnight at 4C. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb Recommended Positive Control: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands Negative Control: Liver Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
