Humanized Di-ubiquitin linked at K63. Epitope: Lys63 ubiquitination site
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFkappaB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Inhibition. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation: Precipitated ubiquitin-containing proteins from HeLa cell lysates. Immunohistochemistry: Ubiquitin (Lsy63 specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Requires HIER using sodium citrate buffer pH6.0 Immunocytochemistry: Ubiquitin (Lsy63 specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat and rabbit brain sections. Inhibition: Preincubation of U1000-06Q with K63- multiubiquitin chains inhibited binding to HeLa cell lysate in Western Blots. Optimal dilutions to be determined by the researcher. Positive Control: Lys63 polyubiquitin chains Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
