Ubiquitin Conjugating Enzyme (E2N, UBE2N, Ubiquitin-conjugating Enzyme E2 N, Ubiquitin-protein Ligase N, Ubiquitin carrier protein N, Ubc13, Bendless-like ubiquitin-conjugating enzyme, BLU) (HRP), Rabbit
Biozol Catalog Number:
USB-U1001-01A-HRP
Supplier Catalog Number:
U1001-01A-HRP
Alternative Catalog Number:
USB-U1001-01A-HRP-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
IHC, WB
Immunogen:
Synthetic peptide corresponding to 15aa located near the human UBC13 carboxy-terminus.
Ubiquitin-conjugating enzyme E2N (UBC13) is a 16kD enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. UBC13 is DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation. Applications: Suitable for use in Immunohistochemistry (paraffin) and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 2ug/ml Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
