Ubiquitin Conjugating Enzyme (E2N, UBE2N, Ubiquitin-conjugating Enzyme E2 N, Ubiquitin-protein Ligase N, Ubiquitin carrier protein N, Ubc13, Bendless-like ubiquitin-conjugating enzyme, BLU) (MaxLight 550), Rabbit
Biozol Catalog Number:
USB-U1001-01A-ML550
Supplier Catalog Number:
U1001-01A-ML550
Alternative Catalog Number:
USB-U1001-01A-ML550-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
IHC, WB
Immunogen:
Synthetic peptide corresponding to 15aa located near the human UBC13 carboxy-terminus.
MaxLight(TM)550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor(TM)546, 555, DyLight(TM)549 , Cy3(TM), TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm), Emission (575nm), Extinction Coefficient 150,000. Ubiquitin-conjugating enzyme E2N (UBC13) is a 16kD enzyme which is an important component of the TLR (Toll-like receptor) and IL-1R signalling pathway. UBC13 is DNA-damage inducible protein which forms part of an enzyme complex, with UEV1A, that indirectly activates TAK1 via the ubiquitination of TRAF-6. Ubiquitin modification of proteins is important for error-free DNA repair and for targeting of short-lived or abnormal proteins for degradation. Applications: Suitable for use in Immunohistochemistry (paraffin) and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 2ug/ml Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
