Purified, E. coli-derived, recombinant human UCH-L1 ( aa2-223).
Ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1), also known as PGP 9.5, is a neuronal and neuroendocrine-specific protein that plays a role in proteosomal protein degradation, which is critical for neuronal health. UCH-L1 is also involved in p27kip1 degradation through its interaction and nuclear translocation with JAB1. UCH-L1 expression is specifically associated with non small-cell lung cancer development and it is found in serum of patients with lung cancer, indicating that it may be important in lung cancer screening and diagnosis. UCH-L1 may also be a useful marker for invasive colorectal cancer. Mutations in the alpha-synuclein and Uch-L1 genes cause autosomal dominant Parkinsons disease (PD). Derangements in the ubiquitin proteasomal pathway may play important roles in the demise of dopamine neurons in PD. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml ELISA (detection): 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
