UCH37, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L5, Ubiquitin Thiolesterase L5, Ubiquitin C-terminal Hydrolase UCH37, CGI-70, AD-019, UCHL5), Rabbit
Catalog Number:
USB-U1005-01L
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| Article Name: | UCH37, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L5, Ubiquitin Thiolesterase L5, Ubiquitin C-terminal Hydrolase UCH37, CGI-70, AD-019, UCHL5), Rabbit |
| Biozol Catalog Number: | USB-U1005-01L |
| Supplier Catalog Number: | U1005-01L |
| Alternative Catalog Number: | USB-U1005-01L-200 |
| Manufacturer: | US Biological |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IHC, WB |
| Immunogen: | Synthetic peptide selected from the N-terminal region of human UCHL5 (KLH). |
| Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230aa human UCHL3 protein is 54% identical to that of UCHL1. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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