Ulex europaeus agglutinin I is a glycoprotein with a molecular weight of 63,000, although multimeric aggregates have been reported. UEA I has two subunits, one of about 31,000D and another of 32,000D. UEA I binds to many glycoproteins and glycolipids containing a -linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inhibiting/Eluting Sugar: 50mM-100mM L-fucose Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Homogeneous by SDS-PAGE
Form:
Supplied as a lyophilized powder.
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