UCP2 (Mitochondrial Uncoupling Protein 2, UCP 2, Solute Carrier Family 25 Member 8, UCPH, SLC25A8), Goat
Biozol Catalog Number:
USB-U1621-03B
Supplier Catalog Number:
U1621-03B
Alternative Catalog Number:
USB-U1621-03B-100
Manufacturer:
US Biological
Host:
Goat
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
Synthetic peptide corresponding to C-DSVKQFYTKGSEH, from human UCP2, at the internal region of the protein (NP_003346.2). Species sequence homology: canine, mouse and rat
White and brown adipose tissues (WAT and BAT, respectively) play a central role in body weight and energy expenditure. WAT is the major site for energy storage via triglyceride synthesis and mobilization via lipolysis. Uncoupling proteins (UCP1-5) are a family of mitochondria transprt proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. BAT is able to dissipate energy as heat via uncoupled mitochondrial respiration by a mitochondrial anion carrier, uncoupling protein 1 (UCP1). UCP1 is predicted to contain 6 transmembrane (TM) domains, a putative purine nucleotide-binding domain (PNBD) and three-mitochondrial energy transfer protein domains (ETPDs). Both amino and C-termini are predicted to be cytoplasmic. Mouse/rat UCP2 is a 309aa (human 309aa, chromosome 7, ~95% homology) mitochondrial uncoupling protein. It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart and muscle). UCP2 may play a critical role in energy balance, body weight and thermoregulation. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:64,000 Western Blot: 0.05-0.1ug/ml, observed in human liver lysates on ~36kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
