A 20-aa peptide sequence within the C-terminus of mouse USF2. Hepcidin (hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25-aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS). USF2 (mouse 342 aa, rat 346 aa, human 346 aa, chromosome 19, ~42kD) are ubiquitously expressed nuclear proteins involved in the transcription of a wide variety of cellular genes. USF1, also known as major late transcription factor (MLTF), has significant sequence homology with USF2. USF2 binds to a symmetrical DNA sequence (E-boxes, 5-CACGTG-3) that is found in a variety of viral and cellular promoters. Effective DNA-binding requires dimerization with another BHLH protein. It binds to DNA as homodimer or heterodimer (USF1/USF2). USF2 is alternatively spliced to two forms: USF2a (rat 346 aa) and USF2b (rat 291 aa. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly purified
Form:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
* VAT and and shipping costs not included. Errors and price changes excepted
