Synthetic peptide corresponding to 11aa near the C-terminus of human URAT1 (KLH). Cellular Localization: Cytoplasmic domain.
Urate, a naturally occurring product of purine metabolism, is a scavenger of oxidants. Urate exists primarily as a weak acid (pKa ~5.75). Urate concentration is quite high (~200-500uM) in human due to loss of uricase gene and the presence of an effective reabsorption system. Recently an OAT-related transporter, URAT1, has been identified in the kidney that acts as urate-anion exchanger and effectively regulates urate levels. URAT1/RST/SLC22A12 (mouse 553-aa, human 555-aa, chromosome 11q13, ~40kD) is ~42% identical with OAT4. Like OAT4, it is predicted to display 12 transmembrane domains with a cytoplasmic N- and C-terminus. URAT1 is prominent in epithelial cells of proximal tubule of the renal cortex. Patients with idiopathic renal hypouricemia (lack of blood uric acid) have defects in URAT1 gene. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml, Control peptide can be used to coat ELISA plates at 1ug/ml. Western Blot: 1-10ug/ml using ECL. Optimal dilutions to be determined by the researcher. Control Peptide: U2005: Urate Transporter 1, Human (URAT1) (Control Peptide) Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
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