Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested. Recommended Dilution: ELISA: 1:4000 to 1:53,000 Optimal dilutions to be determined by the researcher. Biotin/Protein Ratio: 10-20 BAC molecules/goat IgG molecule Label: Biotinamidocaproate N-Hydroxysuccinimide Ester Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
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