Synthetic peptide corresponding to KPRQDTAKSPHRTK from the internal region of human Urocortin 3.
This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:16,000 Western Blot: 1-2ug/ml. In transfected HEK293 transiently expressing full-length human UCN3 (myc and DYKDDDDK tagged), a band of ~19kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Optimal dilutions to be determined by the researcher. Peptide Blocking: U2575-03-P (sold separately): Urocortin 3, corresponding peptide, Peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration is 0.5mg/ml. Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20 minutes. Then incubate and develop the two blots in parallel. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
