KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human USP21. Species sequence homology: bovine, mouse, rat
USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Western Blot: 1:2000 Immunohistochemistry (FFPE): 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.