VANGL2 (van Gogh-like protein 2, also Strabismus and Ltap) is a 65kD member of the Vang family of proteins. It is found in the membranes of embryonic neurons and epithelium where it associates with DVL1-3 and various frizzleds. VANGL2 mutations are associated with neural tube defects. Human VANGL2 is a 521 amino acid (aa) 4-transmembrane protein that contains an N-terminal (aa 1-108) and C-terminal (aa 239-521) cytoplasmic domain. There is a Ser-rich region in the N-terminus (aa 5-20) and a PDZ-binding motif over aa 506-521. Over aa 241-521, human, mouse and rat show total identity in amino acid sequence. Applications: Suitable for use in Direct ELISA and Western Blo. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhVANGL2 is ~ 1ng/well. Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
