A recombinant peptide corresponding to the PK tag fused to a class II-restricted T helper cell epitope.
V0500-00T has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is, gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined). Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[9i129 SV5-Pk5]
Isotype:
IgG1
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, 0.09% sodium azide.
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