The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence. The V5 epitope tag is a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Applications: Suitable for use in RIA, Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:1000-1:5000 Western Blot: 1:1000-1:5000 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0 Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[3H3115 SV5-Pk1]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, 0.09% sodium azide.
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