MaxLight(TM)550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor(TM)546, 555, DyLight(TM)549 , Cy3(TM), TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm), Emission (575nm), Extinction Coefficient 150,000. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence. The V5 epitope tag is a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Applications: Suitable for use in RIA, Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0 Ag14 myeloma cells with spleen cells from Balb/c mice. Recommended Secondary Antibodies: I1904-65W: IgG, F(ab)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab)2X-adsorbed (HRP) Pab Rb xMo Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[3H3115 SV5-Pk1]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight(TM)550.
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