VPS24, Recombinant, Human, aa1-222, His-Tag (Vacuolar Protein Sorting 24 Homolog (S. cerevisiae), Charged multivesicular body protein 3, CGI-149, CHMP3, FLJ38114, NEDF)
Biozol Catalog Number:
USB-V0649-86
Supplier Catalog Number:
V0649-86
Alternative Catalog Number:
USB-V0649-86-100,USB-V0649-86-500
Manufacturer:
US Biological
Category:
Molekularbiologie
VPS24, also known as Charged multivesicular body protein 3(CHMP3), belongs to the vacuolar sorting protein family and function as chromatin modifying proteins. It associates directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. Overexpression of VPS24 strongly inhibits HIV-1 release. Purified by using conventional chromatography. Source: Recombinant corresponding to aa1-222 of human VPS24, fused to His-tag at N-terminal, expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES MDDQEEMEEE AEMEIDRILF EITAGALGKA PSKVTDALPE PEPPGAMAAS EDEEEEEEAL EAMQSRLATL RS Molecular Weight: ~27.2kD (242aa), confirmed by MALDI-TOF Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
