VPS25 (Vacuolar Protein-sorting-associated Protein 25, hVps25, Dermal Papilla-derived Protein 9, ELL-associated Protein of 20kD, ESCRT-II Complex Subunit VPS25, DERP9, EAP20), Goat
Biozol Catalog Number:
USB-V0649-88B
Supplier Catalog Number:
V0649-88B
Alternative Catalog Number:
USB-V0649-88B-100
Manufacturer:
US Biological
Host:
Goat
Category:
Antikörper
Application:
ELISA, IHC, WB
Immunogen:
Synthetic peptide corresponding to C-QPNVDTRQKQ, from human VPS25, at the internal region of the protein (NP_115729.1).
VPS25 is a component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Western Blot: 0.5-1.5ug/ml, observed in human duodenum, kidney and liver lysates on ~20-22kD bands Immunohistochemistry (Paraffin): 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
