Vacuolar Protein Sorting 28, Recombinant, Human (VVPS 28, VPS28, MGC60323, Tumor Susceptibility Gene 101 TSG101, Yeast Class E Protein Vps28p Homolog)
Biozol Catalog Number:
USB-V0650-03
Supplier Catalog Number:
V0650-03
Alternative Catalog Number:
USB-V0650-03-100
Manufacturer:
US Biological
Category:
Molekularbiologie
VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 , expressed in E. coli and purified by using conventional chromatography. Source: Recombinant human VPS28, aa1-221, expressed in E. coli AA Sequence: MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
