VAMP 2, Recombinant, Human (Synaptobrevin 2, Vehicle-associated membrane)
Biozol Catalog Number:
USB-V2024-02L
Supplier Catalog Number:
V2024-02L
Alternative Catalog Number:
USB-V2024-02L-100,USB-V2024-02L-500
Manufacturer:
US Biological
Category:
Molekularbiologie
Synaptobrevin 2 (Vehicle-associated membrane, VAMP2), which is an 18kD integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25. Recombinant protein corresponding to aa1-89 of human VAMP 2, His- tag expressed in E. coli (NP_055047). Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSA TAATAPPAAP AGEGGPPAPP PNLTSNRRLQ QTQAQVDEVV DIMRVNVDKV LERDQKLSEL DDRADALQAG ASQFETSAAK LKRKYW Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, store at -20C. Aliquots are stable for 6 month after receipts at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
13.8
Purity:
95% by SDS PAGE
Form:
Supplied as a liquid in PBS, pH 7.4, 1mM EDTA
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