VAMP B (Vesicle-associated Membrane Protein-associated Protein B, VAMP-B, VAP B, VAPB, VAP-B, ALS8, D2Abb2e, Vamp33b, VAMP C, VAMP-C, VAP C, VAP-C, Vesicle Associated Membrane Protein Associated Protein B and C, Vesicle-associated
Biozol Catalog Number:
USB-V2024-15A
Supplier Catalog Number:
V2024-15A
Alternative Catalog Number:
USB-V2024-15A-100
Manufacturer:
US Biological
Host:
Sheep
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
E. coli-derived, recombinant human VAP-B (aa2-132).
Vesicle-associated membrane protein (VAMP)-associated protein B (VAP-B, also ALS8) is a 33kD, ubiquitously expressed, type IV transmembrane protein and member of the VAP family of proteins. VAP-B may play a role in vesicle trafficking. It is found in plasma and intracellular vesicle membranes as a homodimer and heterodimer with VAP-A, and interacts with VAMP1 and VAMP2. Defects in VAP-B are a cause of amyotrophic lateral sclerosis type 8 and spinal muscular atrophy autosomal dominant Finkel type. Human VAP-B is 243aa in length and contains a 222aa cytoplasmic domain plus a 21aa C-terminal intramembrane segment. Within the cytoplasmic domain is a major sperm protein (MSP) domain (aa8-111). A second isoform, named VAP-C, has a 29aa substitution for aa71-99 plus a deletion of residues corresponding to aa 100-243 in VAP-B. Over aa1-132, human VAP-B is 96% identical to mouse VAP-B. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
