Vanilloid Receptor Like Protein 1 (Vanilloid Receptor-like Protein 1, VRL 1, VRL1, VRL-1, VRL, MGC12549, Osm 9 Like TRP Channel 2, Osm-9-like TRP Channel 2, OTRPC2, Stretch Activated Channel 2B, Stretch-activated Channel 2B, Sac2b
Biozol Catalog Number:
USB-V2075-04E
Supplier Catalog Number:
V2075-04E
Alternative Catalog Number:
USB-V2075-04E-100
Manufacturer:
US Biological
Host:
Goat
Category:
Antikörper
Application:
ELISA
Immunogen:
Synthetic peptide, C-PKEDEDGASEENY, from the C-Terminus of human TRPV2.
This gene encodes an ion channel that is activated by high temperatures above 52 degrees Celsius. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is thought that in other tissues the channel may be activated by stimuli other than heat. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:16000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 monthsat -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See V2075-04E-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
