VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. The majority of VAP-1 is stored within intracellular granules and translocated to the endothelial surface on inflammation. Structurally it belongs to enzymes called semicarbamizide-sensitive amine oxidases. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 possesses both adhesive and enzymatic properties in vitro. VAP-1 mediates leukocyte trafficking to sites of inflammation. Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Inhibition of biological activity. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (frozen): 1:50. Tissue sections were fixed in acetone and incubated with antibody for 20 min at RT. Flow Cytometry: 1:50. Stains the extracellular domain of mouse VAP-1 in CHO cells transfected with mouse VAP-1 domain. Optimal dilutions to be determined by the researcher. Positive Control: Mouse VAP-1-transfected CHO cells Negative Control: Mock-transfected CHO cells Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[9G298 7-88]
Isotype:
IgG2b
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, 0.1% BSA.
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