Sf21-derived recombinant human VAP-1 extracellular domain
Vascular adhesion protein 1 (VAP-1), also known as AOC3 (amine oxidase copper-containing 3) or SSAO (semicarbazide-sensitive amine oxidase) is a 170 kD type II transmembrane glycoprotein consisting of two disulfide-linked 763 amino acid (aa) subunits. It is expressed on endothelium, vascular smooth muscle, and adipocytes. The extracellular domain mediates lymphocyte rolling, adhesion, possibly extravasations during inflammation, and possesses monoamine oxidase activity. Adipocytes can release a soluble form via metalloproteinase cleavage. Human VAP-1 ECD shares 83% and 88% aa sequence identity with mouse and canine VAP-1 ECD, respectively. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human VAP-1. The detection limit for rhVAP-1 is approximately 25 ng/lane under non-reducing and reducing conditions. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human VAP-1. The detection limit for rhVAP-1 is approximately 0.5 ng/well. In this format, this antibody shows approximately 50% cross-reactivity with rmVAP-1. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
