Recombinant protein corresponding to aa48-135 from Varicella Zoster Virus, gE immunodominant region fused to GST-Tag at M-terminal, expressed in E. coli. Amino Acid Sequence: MITNPVRASVLRYDDFHTDEDKLDTNSVYEPYYHSDHAESSWVNRGESSR KAYDHNSPYIWPRNDYDGFLENAHEHHGVYNQGRGIDSGERLMQPTQMSAQEDLGDDTGIHVIPTLNGDDRHKIVNVDQRQYGDVFKGDLNPKPQGQRLIEVSVEENHPFTLRAPIQRIYGVRYTETWSFLPSLTCTGDAAPAIQHICLKHTTCFQDVVVDVDCAENTKEDQLAEISYRFQGKKEADQPWIVVNTSTLFDELELDPPEIEPGVLKVLRTEKQYLGVYIWNMRGSDGTSTYATFLVTWKGDEKTRNPTPAVTPQPRGAEFHMWNYHSHVFSVGDTFSLAMHLQYKIHEAPFDLLLEWLYVPIDPTCQPMRLYSTCLYHPNAPQCLSHMNSGCTFTSPHLAQRVASTVYQNCEHADNYTAYCLGISHMEPSFGLILHDGGTTLKFVDTPESLSGLYVFVVYFNGHVEAVAYTVVSTVDHFVNAIEERGFPPTAGQPPATTKPKEITPVNPGTSPLLRYAAHHHHHH Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Strongly reacts with human VZV positive serum. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
90% by SDS-PAGE and Bradford.
Form:
Supplied as a liquid in 50mM Tris-HCl, 60mM sodium chloride, pH 8.0, 0.25% Sarkosil, 10mM glutathione, 50% glycerol.
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