VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF) (Biotin), Clone: [3F401], Mouse, Monoclonal
Catalog Number:
USB-V2110-05-BIOTIN
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| Article Name: | VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF) (Biotin), Clone: [3F401], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-V2110-05-BIOTIN |
| Supplier Catalog Number: | V2110-05-Biotin |
| Alternative Catalog Number: | USB-V2110-05-BIOTIN-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IHC |
| Immunogen: | Recombinant protein corresponding to human VEGF121. |
| The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms- like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co- operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:50-1:500. Use standard deparaffinization techniques on tissue speciens. Preheat tissues using a citrate buffer, pH 6.0 and high heat epitope retrieval techniques. Note: Do not allow tissues to dry out during the staining procedure. Optimal dilutions to be determined by the researcher. Control: Pancrea, Kidney, Adenocarcinoma Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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