VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF) (FITC), Clone: [3F401], Mouse, Monoclonal
Catalog Number:
USB-V2110-05-FITC
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| Article Name: | VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF) (FITC), Clone: [3F401], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-V2110-05-FITC |
| Supplier Catalog Number: | V2110-05-FITC |
| Alternative Catalog Number: | USB-V2110-05-FITC-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IHC |
| Immunogen: | Recombinant protein corresponding to human VEGF121. |
| The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms- like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co- operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 1:50-1:500. Use standard deparaffinization techniques on tissue speciens. Preheat tissues using a citrate buffer, pH 6.0 and high heat epitope retrieval techniques. Note: Do not allow tissues to dry out during the staining procedure. Optimal dilutions to be determined by the researcher. Control: Pancrea, Kidney, Adenocarcinoma Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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