BALB/C mice were injected with a recombinant VEGF protein.
Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (frozen and paraffin-embedded sections). Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:20-1:50 using ABC method. We recommend incubation period of 30-60 minutes at RT. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to immunostaining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[VG1]
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2.
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