Recombinant protein corresponding to human VEGF165 expressed in E. coli.
Applications: Suitable for use in Neutralization, Immunohistochemistry, FLISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Western Blot: Reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
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