Source: Recombinant corresponding to aa27-190 of mouse VEGF165 expressed in E. coli. Biological Activity: ED50 is less than 2ng/ml as determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.