| Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Study of tumor angiogenesis has led to the identification of several proteins including basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). VEGF acts by interacting with a family of largely endothelial-specific receptor tyrosine kinases that includes VEGFR-1 (flt-1 or fms-like tyrosine kinase 1), VEGFR-2 (flk-1 or fetal liver kinase-1/KDR or Kinase insert domain containing receptor), and VEGFR-3/Flt-4. Disruption of VEGFRs interferes with differentiation of endothelial cells and it is lethal for the embryo. FLT-1 (fms-like tyrosine kinase or VEGF-R1, human 1338aa, rat 1336aa, and mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. It is type 1 membrane protein. It is expressed in normal lung, placenta, liver, kidney, heart, and brain but not in tumor Protein name Vascular endothelial growth factor receptor 1, Synonyms EC 2.7.10.1, VEGFR-1, Vascular permeability factor receptor, Tyrosine-protein kinase receptor FLT, Flt-1, Tyrosine-protein kinase FRT, Fms- like tyrosine kinase 1, Gene name Name: FLT1, FRT Applications: Suitable for use in Western Blot, ELISA, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml (Chemiluminescence) ELISA: 1:10,000-1:100,000, 50-100ng of control peptide/well Immunohistochemistry: 2-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
