Recombinant protein corresponding to the extracellular domain of human VEGFR-2 (KDR).
VEGF, a homodimeric glycoprotein of relative molecular mass 45kD, is the only mitogen that specifically acts on endothelial cells. The importance of VEGF and its receptor system in tumor growth and intervention in this system may provide promising approaches to cancer therapy. VEGF receptor 2 is a member of a receptor tyrosine kinase family. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml Immunohistochemistry (Formalin, frozen): 1-2ug/ml detects VEGFR2 in formalin and acetone fixed tissues. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[FLK-6]
Isotype:
IgG1
Purity:
Purified by affinity chromatography.
Form:
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
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