Recombinant protein corresponding to Tyr25-Asp770 from the extracellular domain of mouse VEGFR3, expressed in Sf21 insect cells
VEGF R3 (Flt4), together with VEGF R1 (Flt1) and VEGF R2 (KDR/Flk1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.In adults, VEGF R3 expression is restricted to endothelial cells of the lymphatic vessels. Mouse VEGF R3 cDNA encodes a 1363aa residue precursor protein with a 24aa residue signal peptide. Mature VEGF R3 has a 751aa residue extracellular domain, a 22aa residue hydrophobic transmembrane domain and a 565aa residue cytoplasmic domain. The polypeptide sequence of murine Flt-4 is 88% identical to the human homologue. VEGF R3 has been reported to serve as a receptor for VEGF-C and VEGF-D. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 1x10e6 cells Optimal dilutions to be determined by the researcher. Excitation: 488nm Emission: 565-605nm Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
