Recombinant protein corresponding to Pro2-Val365 from human Vasohibin isoform 1, expressed in NS0 cells.
Vasohibin is an endothelium-derived negative feedback regulator of angiogenesis. The 365 aa, 44kD human Vasohibin shows 93% aa identity with mouse Vasohibin. Vasohibin has no canonical signal sequence, but the full-length form is only associated with cells, while N-terminally cleaved, active 42kD and 36kD forms are both cell-associated and secreted. Minor amounts of C-terminally cleaved 27kD form are inactive and lack the C-terminal heparin-binding basic domain. Vasohibin expression is upregulated by VEGF and FGF2, but this can be modulated by IL-1, TNFalpha or IFNy. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhVasohibin is ~ 1ng/well. Western Blot: 1ug/ml detection limit for rh Vasohibin is ~ 25ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
