VAV3 (Vav 3 Oncogene, Oncogene VAV23 Guanine Nucleotide Exchange Factor, Gef, GDP/GTP Exchange Factor, Gef), Rabbit
Catalog Number:
USB-V2121-15C
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| Article Name: | VAV3 (Vav 3 Oncogene, Oncogene VAV23 Guanine Nucleotide Exchange Factor, Gef, GDP/GTP Exchange Factor, Gef), Rabbit |
| Biozol Catalog Number: | USB-V2121-15C |
| Supplier Catalog Number: | V2121-15C |
| Alternative Catalog Number: | USB-V2121-15C-200 |
| Manufacturer: | US Biological |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IHC, IP, WB |
| Immunogen: | Synthetic peptide corresponding to aa566-591 (NSGEQGTLKLPEKRTNGLRRTPKQVD) of human Vav3 (KLH). |
| Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases. The VAV family has currently three members in mammalian cells (VAV 1, VAV 2, and VAV 3). VAV 1 contains multiple protein binding motifs, indicating a role for VAV 1 as an adaptor protein linking cell surface receptors to downstream signaling proteins. VAV 1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NF(kappa)B, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN lambda, and TNF alpha. VAV 3 is also involved in activating JNK, p21-activated kinase (PAK) and PLC lambda, implicating a critical role in cytoskeletal rearrangement. VAV 3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro. Tyrosine 173 is the regulatory phosphorylation site of VAV 3. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detected Vav3 in RIPA lysates from HeLa and Jurkat cells. Immunoprecipitation: Reported to immunoprecipitate Vav3 from Jurkat cell lysate and SU86.86 pancreatic tumor cell lysate. Immunocytochemistry: Reported to detect Vav3 in Jurkat and SU86.86 pancreatic tumor cells. Immunohistochemistry: Reported to detect Vav3 in pancreatic adenocarcinoma sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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